AnalyzER
Overview
| Measured variables | Morphology of endoplasmic reticulum, Dynamics of endoplasmic reticulum |
|---|---|
| Operating system | Windows |
| Licence | Open source |
| Automation level | Semi-automated |
| Plant requirements | Any |
| Export formats | xlsx |
| Other information |
Scientific article(s)
Quantitative analysis of plant ER architecture and dynamicsCharlotte Pain, Verena Kriechbaumer, Maike Kittelmann, Chris Hawes, Mark FrickerNature Communications, 2019 View paper
Description
The aim of the AnalyzER program is to quantify: The length, width, morphology and protein distribution along the ER tubules - The degree and branch angles at junctions (nodes) in the tubular network - The size, shape and protein distribution in cisternal sheets and around the perimeter of the cisternae - The topological organisation of the tubular and cisternal network determined using graph-theoretic metrics - The distribution of immobile nodes, tubules and cisternae using persistency mapping - The local speed and direction of movement of tubules and cisternae using optical flow - The size and shape of the polygonal regions enclosed by the network.