FRETENATOR2

Jim Rowe

Overview

Measured variables	Ratiometric measurements of
Operating system	Windows
Licence	Open source
Automation level	Semi-automated
Plant requirements	Arabidopsis
Export formats	tif, csv
Other information

Scientific article(s)

Quantifying Phytohormones in Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset
James H. Rowe,Annalisa Rizza,Alexander M. Jones
Methods in Molecular Biology, 2022 View paper

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Description

FIJI plugin for full 3D segmentation of images of nuclear localised ratiometric biosensors and to calculate emission ratios on a per nucleus basis allowing in-depth analysis of biosensor data. Compatible with windows, mac, linux. Discrete graphics card with enough video memory recommended. The old version is available on the following website : https://github.com/JimageJ/ImageJ-Tools?tab=readme-ov-file